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Environmental stresses are the one of the main reasons for the decline of crop production worldwide. In the past years, a major focus has been on improving plant species and their tolerance towards these stresses but not much has been achieved because of the limited knowledge of the gene/network of genes that might be involved in providing tolerance to such multiple abiotic stresses. Recently, members of Stress Associated Protein (SAP) family in plants have been shown to impart tolerance to multiple abiotic stresses. There are 14 SAP genes in Arabidopsis thaliana and these proteins contain A20, AN1 and C2H2 zinc finger domains. AtSAP13, a member of the SAP family, carries two AN1 zinc finger domains and an extra Cys2-His2 domain. AtSAP13 showed differentially regulation in response to multiple abiotic stresses such as toxic metals arsenic (As), cadmium (Cd), and zinc (Zn), drought, and salt. When overexpressed in Arabidopsis and Brassica juncea, it showed strong tolerance to these stresses. However, the mode of action of this SAP member in providing tolerance to multiple abiotic stresses is largely unknown. In-silico analysis of the promoter sequences upstream of ATG start codon of AtSAP13 using PLACE database predicted the presence of various abiotic stress related cis regulatory elements. We hypothesized that the expression of AtSAP13 gene might be regulated via the interaction of cis-elements present in the AtSAP13 promoter with abiotic stress related trans factors via protein-DNA interactions under different abiotic stresses. Through yeast one hybrid assay (Y1H), we have proved this hypothesis and identified several transcription factors such as DREB, ERE, ZIP, HSE etc that are interacting with the AtSAP13 promoter. These interactions were analyzed through Electrophoretic mobility shift assay (EMSA) to understand the molecular and biochemical functioning of AtSAP13.
AN1 zip
This is a list of postcode districts in the United Kingdom and Crown Dependencies. A group of postcode districts with the same alphabetical prefix is called a postcode area. All, or part, of one or more postcode districts are grouped into post towns.[1]
Until 1996, Royal Mail required counties to be included in addresses, except for 110 of the larger post towns. For these "special post towns", the former postal county is shown in brackets below. Since 1996, counties are not required for any address.
One Earth is dedicated to mobilizing philanthropic capital to protect the ecosystems and peoples of Antarctica. Visit the Project Marketplace to explore projects in this realm that need your support. Learn more about each of the Continental Antarctica ecoregions below.
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In the new version of Gangstar Vegas, there will be an event that any player will enjoy because of the interesting gameplay that is mind control. The goal in this mode is quite simple: you will use a vehicle capable of mind control of others and begin killing marked enemies that you will easily recognize. So you will use tactics to take them down and get impressive rewards in this event.
The game has prepared for you a dedicated vehicle to mind control the different characters in the game. In this game, you need to pay attention to that you will need to choose the correct characters to defeat your enemies. At the same time, most of the time, you will select the targets that you need to destroy because they have weapons and quickly take down other enemies. In addition, you can also aim at anyone you see and do not let the controlled character get close to your car.
The story that players experience in Gangstar Vegas revolves around Jason, an MMA athlete. Everything was normally going until mafia boss Don Frank Veliano appeared before Jason and asked for favors. Specifically, you are forced to fake defeat to your opponent in his next match. Of course, because of the influence of the Mafia boss, Jason followed suit, but things were not as he imagined.
If you are a fan of the hit GTA series, you will undoubtedly find similar points appearing in Gangstar Vegas. It is an open world where you can easily find a variety of enjoyable experiences in an open world. You can interact with what you want and experience the vastness that this game possesses. In addition, it also creates much different gameplay that you will choose for yourself depending on your preferences.
The first experience will usually be associated with the open-world when the environment element is fully designed. You will quickly have a vehicle and go to any location you desire to satisfy your desires.
The second type of experience where you will spend time completing objectives is doing missions. You will try to complete the story-related quests to find out what happens after Jason returns to work for Montello after escaping from Frank. At the same time, these missions are completely intense and impressive as you will be able to confront the mafia or try to drive quickly to the specified location.
Besides the primary experience that you can find in Gangstar Vegas, you also find some other exciting gameplay. You can go into places where bloodthirsty zombies are always trying to catch and attack you until you are motionless. Of course, you will also be equipped with weapons to fight them. In addition, you can also join a gang to experience gang battles against other players with the weapons you have.
The number of weapons and items you receive is entirely diverse that you can unlock, use, and equip your character when starting with each different mode. At the same time, event items always attract the attention of players.
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Feature papers represent the most advanced research with significant potential for high impact in the field. A FeaturePaper should be a substantial original Article that involves several techniques or approaches, provides an outlook forfuture research directions and describes possible research applications.
Xie, H.; Yang, Q.; Wang, X.; Schläppi, M.R.; Yan, H.; Kou, M.; Tang, W.; Wang, X.; Zhang, Y.; Li, Q.; Dai, S.; Liu, Y. Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance. Int. J. Mol. Sci. 2022, 23, 11551.
Xie H, Yang Q, Wang X, Schläppi MR, Yan H, Kou M, Tang W, Wang X, Zhang Y, Li Q, Dai S, Liu Y. Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance. International Journal of Molecular Sciences. 2022; 23(19):11551.
Xie, Hao, Qiangqiang Yang, Xiaoxiao Wang, Michael R. Schläppi, Hui Yan, Meng Kou, Wei Tang, Xin Wang, Yungang Zhang, Qiang Li, Shaojun Dai, and Yaju Liu. 2022. "Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance" International Journal of Molecular Sciences 23, no. 19: 11551.
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